Supplementary Figure 2: DNA binding of E2f3 and Myc in wild type crypts. | Nature Cell Biology

Supplementary Figure 2: DNA binding of E2f3 and Myc in wild type crypts.

From: Redeployment of Myc and E2f1–3 drives Rb-deficient cell cycles

Supplementary Figure 2

(a) IHC staining of E2f3 using the antibody (Millipore; 05-551) in control, E2f3a−/−, E2f3b−/− and Ah-cre;E2f1−/−;E2f2−/−;E2f3loxP/loxP intestines. Data are representative images from n = 2 mice per genetic group. E2f3b−/− but not E2f3a−/− intestines show positive-stained cells, indicating the specific recognition of E2f3a isoform by this antibody. Note the non-specific staining of blood cells. (b) Heatmap representation of differentially expressed genes in wild type crypts (n = 5) versus wild type villi (n = 5). P < 0.01, empirical Bayes method. (c) ChIP-exo-seq track examples showing E2f3 and Myc binding to selected G1-S and S-G2 related genes in wild type crypts. Examples are derived from pooled crypts (n = 32 mice). Scale bars in a represent 25 μm. Scale bars in c represent 1kb.

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