Supplementary Figure 1: Loss of E2f1-3 and/or Myc in crypts.
From: Redeployment of Myc and E2f1–3 drives Rb-deficient cell cycles
(a) Dual staining of EdU (red) and geminin (green, included here for the composition of merged images) in crypts from intestines harvested 2 days after induction of Ah-cre expression. Nuclei were stained with DAPI (blue). Data are representative images from n = 3 mice per genetic group. (b) Quantification of EdU and geminin staining. Data presented as mean ± s.d., n = 3 mice per genetic group. (c) IHC staining of Myc in intestines harvested 2 days after induction of Ah-cre expression. Data are representative images from n = 3 mice for each genetic group. (d) Alcian Blue staining for goblet cells (blue) and IF staining of lysozyme for paneth cells (red) in intestines harvested 2 days after induction of Ah-cre expression. Nuclei were counterstained with Nuclear Fast Red or DAPI. Data are representative images from n = 3 mice per genetic group. Scale bars in a, c and d represent 50 μm.
