Supplementary Figure 2: ISC tumors induce EGF, insulin, PDGF-VEGF and Wnt signaling in the niche.

(a) Mean normalized expression values (RPKM, log₂) of spi determined by mRNA sequencing of sorted midgut cell types from n = 2 independent experiments. (b) Mean fold change (log₂) with s.e.m. from n = 4 independent experiments of EGFR ligand mRNA levels determined by qPCR on midguts bearing 7 day neur^IF65/− clones. Fold changes were normalized to control midguts bearing 7 day wild-type clones. (c,d) Mean normalized expression (NE) value (RPKM, log₂) from n = 2 independent experiments of other midgut signaling factors determined by mRNA sequencing of midguts expressing GFP (control, red) or GFP and N^RNAi (tumorous, blue) with esg^ts for 3 days (c). The adjusted fold change in gene expression in tumorous midguts (blue), normalized to control midguts (red), is indicated. Benjamini–Hochberg adjusted P-value for Ilp3 is 2.47e-35; Pvf2, 4.00e-46 and wg, 0.0016. The mean NE value (RPKM, log₂) from n = 2 independent experiments of midgut signaling factors determined by mRNA sequencing of esg⁺ cells sorted from control midguts expressing GFP (red) or from tumorous midguts expressing GFP and N^RNAi (blue) with esg^ts for 3 days (d). NS, not significant.