Supplementary Figure 3: Tumor frequency and incidence after inhibiting apoptosis, JNK and Yki activity and cytokine expression in enterocytes.

(a) 8 midgut regions used to determine the frequency of neur^IF65/− tumor incidence (b) and tumor size (c) along the midgut anterior (A)–posterior (P) axis. (b) Mean percent of total neur^IF65/− tumors (n = 45) found in each of 8 midgut regions. (c) Mean number of cells per neur^IF65/− tumor (n = 45) with s.e.m. found in each of 8 midgut regions. (d,f,h,j,l) Mean tumors per midgut with s.e.m. 7 or 8 days after tumor induction in midguts expressing GFP (n = 6 midguts) or GFP and p35 (n = 12 midguts) (d), GFP (n = 16 midguts) or GFP and bsk^K53R (n = 17 midguts), puc (n = 6 midguts), bsk^RNAi (n = 16 midguts) or hep (n = 28 midguts) (f), GFP (control, n = 16 midguts) or GFP and wts (n = 19 midguts) or yki^RNAi (n = 9 midguts) (h), GFP (control, n = 17 midguts) or GFP and upd2^RNAi(1) (n = 11 midguts), upd2^RNAi (2) (n = 9 midguts), upd3^RNAi(1) (n = 12 midguts) or upd3^RNAi (2) (n = 10 midguts) (j), and GFP (control, n = 25 midguts) or GFP and Ras^{V 12} (n = 26 midguts) (l) in ECs with Myo1A^ts. (e,g,i,k,m) Mean percent flies with tumors with s.e.m. 7 or 8 days after tumor induction in midguts expressing GFP or GFP and p35 (e), GFP or GFP and bsk^K53R, puc, bsk^RNAi or hep (g), GFP or GFP and wts or yki^RNAi (i), GFP or GFP and upd2^RNAi(1), upd2^RNAi(2), upd3^RNAi(1) or upd3^RNAi (2) (k), and GFP or GFP and Ras^{V 12} (m) in ECs with Myo1A^ts. In b,c, tumors pooled from 2 independent experiments; in d, f, h, j and l, midguts were pooled from 3 independent experiments; in e, g, i, k, and m, mean determined from n = 3 experiments.