Supplementary Figure 4: ISC tumour growth induces enterocyte detachment and loss.

(a) DNA (a, blue) in midguts containing neur^IF65/− tumours (yellow dashed line) amongst ECs expressing GFP (a′, green) with Myo1A^ts for 8 days. Arrowheads indicate ECs overlying tumours (a,a′). (b,c) Mean normalized expression (NE) value (RPKM, log₂) from n = 2 independent experiments of pro-apoptotic factors (hid, rpr, grim and skl) determined by mRNA sequencing of midguts expressing GFP (control, red) or GFP and N^RNAi (blue) with esg^ts for 3 days (b). The adjusted fold change in gene expression in tumorus midguts (blue), normalized to control midguts (red), is indicated. The Benjamini–Hochberg adjusted P-value for grim is 5.86e-127. The mean NE value (RPKM, log₂) from two independent experiments of apoptosis factors determined by mRNA sequencing of esg⁺ cells sorted from control midguts expressing GFP (red) or from tumorous midguts expressing GFP and N^RNAi (blue) with esg^ts for 3 days (c). NS, not significant. (d) Transmission electron micrograph of an apoptotic EC (anterior) from midguts expressing GFP and N^RNAi with esg^ts for 3 days. White arrowheads highlight abnormal EC microvilli. Scale bars in a–a′, 30 μm; in d, 2 μm.