Supplementary Figure 7: Oncogenic Ras^{V 12} expression in ECs induces JNK activity and stimulates ISC tumour growth.

(a–c) β-galactosidase (a–c; a′–c′, red) in midguts bearing puc-lacZ and expressing GFP (a′,b′,c′; green) alone for 5 days (a) or GFP and hep^Act for 2 days (b) or GFP and Ras^{V 12} for 5 days (c) with Myo1A^ts. (d) Mean number of phosphorylated histone H3 Ser10 positive cells per midgut with s.e.m. in flies expressing GFP alone (control, n = 11 midguts), GFP and hep (n = 9 midguts, Mann–Whitney: p = 0.0064) or Ras^{V 12} (n = 15 midguts, Mann–Whitney: P < 0.0001) for 7 days or GFP and hep^Act (n = 11 midguts) for 2 days with Myo1A^ts. (e) Cells per neur^IF65/− tumour with mean (red line) and s.e.m. amongst ECs expressing GFP (control, n = 56 tumours from 25 midguts, skewness = 2.929, kurtosis = 12.39) or GFP and Ras^{V 12} (n = 35 tumours from 26 midguts, Mann–Whitney: p = 0.0004, skewness = 2.049, kurtosis = 4.029) with Myo1A^tsfor 7 days. In d, midguts pooled from 2 independent experiments; in e, from 3 independent experiments. DNA in a′, b′ and c′ (blue). Scale bars in a–c′ are 45 μm.