Supplementary Figure 5: CapZ regulates actin puncta formation.

(a) NRK cells were transfected with nonspecific- (NS) or Capzb-RNAi. Cells were starved for 0 or 4 h and stained with phalloidin and antibody to LC3. Scale bar, 5 μm. (b) Cells from (a) were quantified for phalloidin-positive LC3 puncta. (n = 3 independent experiments; 50 cells were assessed per independent experiment.) Data represent mean ± s.d. ^∗∗P < 0.01 (two-tailed t-test). (c) Stable Capzb knockdown NRK cells were transfected with CapZβ-Myc, then starved for 0 or 4 h and stained with antibody to LC3 and phalloidin. Regions outlined with white dashed lines are magnified. Scale bars in full panels and zoomed panels correspond to 5 μm and 1 μm, respectively. (d) Cells from (c) were quantified for phalloidin-positive LC3 puncta. (n = 3 independent experiments; 100 cells were assessed per independent experiment). Data represent mean ± s.d. ^∗∗∗P < 0.001 (two-tailed t-test).