Supplementary Figure 1: Actin puncta are co-localized with LC3 puncta. | Nature Cell Biology

Supplementary Figure 1: Actin puncta are co-localized with LC3 puncta.

From: CapZ regulates autophagosomal membrane shaping by promoting actin assembly inside the isolation membrane

Supplementary Figure 1

(a) GFP-LC3 expressing NRK cells were starved for 4 h and time-lapse images were acquired with a NIKON A1 confocal microscope. The number of autophagosomes at the indicated time points was quantified. (n = 3 independent experiments; 50 cells per time point were assessed per independent experiment.) Data represent mean ± s.d. (b) NRK cells were starved for 4 h then stained with phalloidin (to detect polymerized actin) and antibody to LC3. Regions of LC3 puncta that colocalize with phalloidin are outlined with white dashed lines and are magnified to the right. Scale bars in full panels and zoomed panels correspond to 5 μm and 1 μm, respectively.

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