Supplementary Figure 5: Mature adipocyte specific inducible deletion of C/EBPα alters whole body lipid metabolism during HFD feeding.

a,b. Lipoprotein profiles determined using pooled serum samples by FPLC fractionation from Adn-C/EBPα^−/− mice and their control littermates at the 4th week of doxycycline HFD feeding. Triglyceride (a) and cholesterol (b) concentrations were measured in each indicated fraction corresponding to VLDL, IDL/LDL and HDL, respectively (left). The relative triglyceride content in VLDL fractions (a) and cholesterol content in HDL fractions (b) are also shown using the areas under curve from the FPLC profiles (right). n = 6 male mice per group. This experiment is representative of two independent experiments. c. Triglyceride clearance test (20% intralipid, 10 μl per gram of body weight, single gavage) in Adn-C/EBPα^−/− mice and their control littermates. n = 9 male mice (HFD control group), n = 5 male mice (HFD Adn-C/EBPα^−/− group). **, P < 0.001, compared to HFD control group. Two-way ANOVA. This data is from a single experiment. d. Triglyceride secretion rate were measured in Adn-C/EBPα^−/− mice and their control littermates after tyloxapol administration (through tail-vein injection, 600 mg kg⁻¹ body weight). Blood triglycerides were measured at the time points as indicated (left), and the VLDL secretion rate (right) was derived from the slope of the line using least squares regression. n = 3 male mice (HFD control group), n = 4 male mice (HFD Adn-C/EBPα^−/− group). **, P = 0.002, < 0.001 for triglyceride levels at 3 h and 4 h; Two-way ANOVA. *, P = 0.008 for VLDL secretion rate, compared to HFD control group. Student’s t-test. This data is from a single experiment. e–g. Circulating NEFA (e), triglyceride (f) and glucose (g) in Adn-C/EBPα^−/− mice and their control littermates at 5th week of doxycycline HFD feeding after β3 agonist treatment (1 mg/kg, body weight). n = 7 male mice (HFD control group), n = 12 male mice (HFD Adn-C/EBPα^−/− group). *, P = 0.02 for triglyceride level at 120 min; *, P = 0.04, 0.02 for glucose levels at 30 and 60 min, compared to HFD control group. Two-way ANOVA. This data is from a single experiment. h,i. Hepatic triglyceride (TG) (h) and cholesterol (CHO) (i) levels in Adn-C/EBPα^−/− mice and their control littermates after 4 weeks of doxycycline HFD feeding. n = 12 male mice (HFD control group), n = 11 male mice (HFD Adn-C/EBPα^−/− group). This data is from a single experiment. All data represent the mean ± s.e.m. j–m. Physical activity (j), oxygen consumption (k), RER (l) and food intake (m) were determined for Adn-C/EBPα^−/− mice and their control littermates during the 7th week of doxycycline HFD feeding. n = 6 male mice per group. XA: X beam activities; YA: Y beam activities; CenA: Central beam activities. This data is from a single experiment.