Supplementary Figure 6: Inducible deletion of C/EBPα in mature adipocytes during HFD feeding alters ceramide content and expressions of key transcription factors, adipokines and enzymes in WAT.
From: Distinct regulatory mechanisms governing embryonic versus adult adipocyte maturation
a–i. Sphingoid base contents were measured by LC/MS/MS method in liver (a), sWAT (d) and eWAT (g) of Adn-C/EBPα−/− mice and their control littermates after 1 month of doxycycline HFD feeding. Ceramides contents were measured by LC/MS/MS method in liver (b), sWAT (e) and eWAT (h) of Adn-C/EBPα−/− mice and their control littermates after 1 month of doxycycline HFD feeding. Sphingomyelin and Ceramide content was measured by LC/MS/MS method in liver (c), sWAT (f) and eWAT (i) of Adn-C/EBPα−/− mice and their control littermates after 1 month of doxycycline HFD feeding. n = 11 male mice (HFD control group), n = 12 male mice (HFD Adn-C/EBPα−/− group). Data represent the mean ± s.e.m. (a) **, P = 0.004; (b) from left to right: *, P = 0.01, **, P = 0.007, *, P = 0.04; (d) from left to right: *, P = 0.04, **, P < 0.001; (e) from left to right: **, P < 0.001, **, P < 0.001, **, P < 0.001, **, P < 0.001, **, P < 0.001, *, P = 0.03, **, P = 0.006, **, P < 0.001, *, P = 0.01; (f) from left to right: *, P = 0.02, **, P < 0.001; (g) from left to right: **, P < 0.001, *, P = 0.02, **, P < 0.001; (h) from left to right: *, P = 0.04, *, P = 0.02, **, P < 0.001, **, P < 0.001, **, P = 0.002, *, P = 0.03, *, P = 0.02; (i) *, P = 0.01. All compared to HFD control group. Student’s t-test. This data is from a single experiment. j–l. mRNA levels of key transcription factors were measured by qPCR, including PPARγ, SREBP1c, SREBP2, ChREBP, LXRα, LXRβ and PGC1-α (j); adipocyte markers adiponectin (ADPN), resistin, CD36, FATP1, CPT1α and Acox1 (k); lipogenic enzymes FAS, ACC, SCD-1 and Elovl6 (l) in sWAT of Adn-C/EBPα−/− mice and their control littermates after 4 weeks of doxycycline HFD feeding. n = 13 male mice per group. Data represent the mean ± s.e.m. *, P = 0.04 for Elovl6; **, P < 0.001 for PPARγ, LXRα, adiponectin, resistin, CD36 and SCD-1. All compared to HFD control group. Student’s t-test. This data is from a single experiment.
