Supplementary Figure 8: The possible synergy between OTUD3 and USP13, and the phenotype of OTUD3 transgenic mice.

a, Akt phosphorylation was analysed in MCF7 cells stably expressing the indicated shRNA. b–d, The MCF7 cells expressing OTUD3 WT or mutants were measured for cell proliferation (b, two-way ANOVA test), anchorage-independent growth in soft agar (c, Student’s t-test) and transwell migration assay (d, Student’s t-test). Data are shown as mean ± s.d. n = 3 independent experiments. **: P < 0.01, *: P < 0.05. e, Representative bright-field imaging of the tumors. MCF7cells stably expressing indicated shRNA were implanted into a subcutaneous site in the skin on one flank of athymic nude mice (n = 8). On 4 weeks, mice receiving transplants of indicated cells were sacrificed. Scale bar, 1 cm. f, Whisker plots showed the distribution of the tumor weights (n = 8 tumors from 8 mice). Data were shown as mean ± s.d. and analysed using Kruskal-Wallis test. g, WT and TG MEFs were serum-starved and treated with EGF for various times. Quantification of the phosphorylation levels of Akt, p38 and Erk protein relative to total Akt, p38 and Erk protein levels for Fig. 5i. Data are shown as mean ± s.d. n = 3 independent experiments (two-way ANOVA test). h, The mating strategy of OTUD3 TG mice crossing with MMTV-PyMT mice. Panels a,g are representative results of 3 or more independent experiments. Statistics source data can be found in Supplementary Table 4. Uncropped images of blots are shown in Supplementary Fig. 9.