Supplementary Figure 9: OTUD3 E86K mutation in OTU domain leads to PTEN protein destabilization and increased tumorigenesis.

a, Sequencing the region encoding OTUD3 OTU domain from 50 cases of Chinese breast cancer patients. Breast cancer tissues (n = 50) and the corresponding adjacent tissues (n = 50) were collected and subjected to RNA extraction and reverse transcription. The cDNAs were then sequenced. If the original sequencing results displayed heterozygous peaks, the corresponding cDNAs were ligated with pGEM-T vector (Promega) and transformed into the competent bacteria. Then the colonies were sent out for sequencing. b, The mutation sites of OTUD3 in domain structure. Amino acid sequence alignment spanning OTUD3 R79 and E86 across species. c, Half-life of PTEN in the presence or absence of ectopic OTUD3 mutants. Quantification of the PTEN protein levels relative to β-actin for Fig. 6d (**: P < 0.01, two-way ANOVA test). Data are depicted as bar graphs with mean ± s.d. n = 3 independent experiments. Statistics source data can be found in Supplementary Table 4.