Supplementary Figure 2: Cut7 motor activity is essential for its kinetochore function.

(a) The signals of CFP^−Cnp3C, Cut7d-CFP^−Cnp3C and Cut7*d-CFP^−Cnp3C were observed in the indicated cells. The centromeres were visualized by cen2-GFP. (b) Serial dilution assay showing that motor-dead version of Cut7d (Cut7*d) cannot suppress the TBZ hypersensitivity of mad1-KAKA cells (TBZ 15 μg ml⁻¹). (c) The indicated cells were monitored for cen2-GFP segregation in bi-nucleate cells as in Fig. 1b. Over 500 cells per strain were analysed. Error bars, s.e.m. for n = 3 independent experiments. (d) Serial dilution assay showing that cut7d^−cnp3C suppresses the TBZ hypersensitivity of mad1Δ cells (10 μg ml⁻¹). (e) The indicated cells were monitored for cen2-GFP segregation in bi-nucleate cells as in Fig. 1b (over 500 cells per strain were analysed). Error bars represent s.e.m. for n = 3 independent experiments. (f) Serial dilution assays showing that centromere targeting of Cut7 can suppress the TBZ hypersensitivity of bub1Δ, bub3Δ, and mph1Δ cells. Scale bars, 4 μm.