Supplementary Figure 3: Deletion of counteracting kinesins enhances Cut7-mediated chromosome gliding.
From: Mad1 promotes chromosome congression by anchoring a kinesin motor to the kinetochore
Chromosome gliding assays were performed as in Fig. 5c–e in the absence of minus-end directed kinesin-14 motors (Pkl1 and Klp2 in fission yeast). Kinesin-14 motors were implicated in chromosome movement toward the spindle poles in several organisms including fission yeast37. Note that greater chromosome gliding observed in Kinesin-14 deleted cells, supporting the idea that Cut7 promotes plus-end directed gliding. Around 20 cells per strain were analysed. Scale bars, 2 μm.
