Supplementary Figure 6: The O-GlcNAcylation of MIF Inhibits EGF-induced Brain Tumorigenesis, But Has No Effect On EGFRvIII-induced Tumor Growth.
From: Secreted and O-GlcNAcylated MIF binds to the human EGF receptor and inhibits its activation
A total of 5 × 105 U87 cells with or without MIF shRNA and EGFR shRNA (a) or U87 cells with or without WT Flag-MIF or Flag-MIF S112/T113A expression (b) or U87/EGFRvIII cells with or without expression of MIF WT or MIF S112/T113A mutant (c) were intracranially injected into athymic nude mice (n = 8 mice per group). After 2 weeks, the mice were euthanized and examined for tumor growth. Hematoxylin and eosin-stained coronal brain sections show representative tumor xenografts. Tumor volumes were measured by using length (a) and width (b) and calculated using the following equation: V = ab2/2. Data represent the means ± SD (n = 8 mice per group). A two-tailed Student’s t test was used. *, P < 0.05; NS, not significant.
