Supplementary Figure 1: mTOR inhibition regulates the SASP.
From: mTOR regulates MAPKAPK2 translation to control the senescence-associated secretory phenotype
(a) mTOR inhibition regulates the SASP in different fibroblasts strains. BJ ER:RAS and HFFF2 ER:RAS cells were generated and induced with 4OHT to activate ER:RAS in the presence of 10 nM rapamycin, 25 nM Torin1 or 0.5 nM NVP-BEZ235. Seven days after 4OHT induction, RNA was collected and qPCRs performed to detect the expression of selected SASP components. Data are mean ± s.d. from n = 3 independent experiments. Student’s t-test was performed to compare the different treatments to the control (R + DMSO) with ***P < 0.001. V, cells expressing an empty vector; R, cells infected with pLNC-ER:RAS. (b,c) mTOR inhibition (b) or mTOR knockdown (c) decreases phosphorylation of S6 (pS6S240/S244) and eIF4EBP1 (p4EBP1T37/T46) proteins and inhibits the induction of the SASP. Indicated immunostainings were performed 7 days after 4OHT induction. Fold changes of normalised values and representative images are shown. Error bars represent the ±s.d. from n = 4 independent experiments. Student’s t-test was performed to compare treated/shMTOR cells to the control senescent cells. ***P < 0.001. Scale bar, 30 μm. (d) Venn diagrams representing the overlap and significance for the SASP components downregulated or upregulated by multiple mTOR inhibitors and mTOR knockdown. The significance of overlap was computed using hypergeometric test. **P < 0.01; *P < 0.05. For raw data, see Supplementary Table 7.
