Supplementary Figure 10: Exogenous mammalian bile acids enhance the induction of pgp-5::gfp in response to mild translation inhibition. Mammalian bile acids do not induce pgp-5::gfp expression in the absence of ribosomal defects. Scale bar, 50 μm. | Nature Cell Biology

Supplementary Figure 10: Exogenous mammalian bile acids enhance the induction of pgp-5::gfp in response to mild translation inhibition. Mammalian bile acids do not induce pgp-5::gfp expression in the absence of ribosomal defects. Scale bar, 50 μm.

From: Lipid signalling couples translational surveillance to systemic detoxification in Caenorhabditis elegans

Supplementary Figure 10

(a) Quantification of gfp fluorescence in animals treated with mammalian bile acids. Error bars represent SD. Unpaired t-test. n = 10 worms for each condition. ns no significant difference. Data represent one out of two independent experiments. (b) Exogenous mammalian bile acids enhance the induction of pgp-5::gfp expression in response to mild translational defect in dilute rpl-7 RNAi-treated animals. Fold change was calculated in comparison to pgp-5::gfp carrying animals. Error bars represent SD. One-way ANOVA; ∗∗∗P < 0.001. ns, P > 0.05. n = 10 worms for each condition. Data represent one out of two independent experiments. (c) Mammalian bile acids are sufficient to induce pgp-5::gfp expression in the vhp-1(sa366);pgp-5::gfp strain without ribosomal defects. Scale bar, 50 μm. (d) Mammalian bile acids do not induce hsp-4::gfp (a ER stress response chaperone gene) in response to low doses of DTT. Scale bar, 50 μm. (e) Mixed bile acid treatment or Kocuria rhizophila feeding does not induce gpdh-1::gfp expression. Error bars represent SD. One-way ANOVA. ns, P > 0.05. n = 10 worms for each condition. Data represent one out of two independent experiments. (f) Mammalian bile acids enhance the response to mild translational defect in dilute eft-4 RNAi-treated animals to induce pgp-5::gfp expression. Scale bar, 50 μm. (g) Lipid extract from eft-4RNAi, iff-2 RNAi or rpl-7 RNAi enhance the mild translational defect in dilute eft-4 RNAi-treated animals to induce pgp-5::gfp expression. Fold change was calculated in comparison to pgp-5::gfp carrying animals. Error bars represent SD. One-way ANOVA; ∗∗∗P < 0.001. ns, P > 0.05. n = 10 worms for each condition. Data represent one out of two independent experiments. (h) pgp-5::gfp expression in wild type animals is not induced by co-culturing with a large excess of eft-3(q145) mutant animals each experiencing germline-translation defects and thus sterility, but not carrying pgp-5::gfp. Thus there is no pheromone for translational stress or bile acid signaling between animals. Scale bar, 50 μm. (i) daf-12 nuclear hormone gene activity is not necessary for pgp-5::gfp induction by hygromycin and addition of the ligand for DAF-12, dafachronic acid, is not sufficient to induce pgp-5::gfp absence of hygromycin. n = 20 worms for each condition. Data represent one out of two independent experiments. (j) Quantification of the GFP fluorescence in worms growing on lawns of Paenibacilli, Kocuria or Alcaligenes bacteria that disrupt induction of pgp-5::gfp in response to germline translation defects in the eft-3(q145);pgp-5::gfp strain, compared to growth on control E. coli OP50. Error bars represent SD. One-way ANOVA; ∗∗∗P < 0.001. n = 10 worms for each condition. Data represent one out of two independent experiments. (k) Animals growing on lawns of Paenibacilli, Kocuria or Alcaligenes bacteria show normal induction of hsp-4::gfp in response to ER stress. Scale bar, 50 μm. (l) Kocuria anti-translation surveillance activity is live cell-associated, inactivated by heat and requires continued exposure to Kocuria. n = 30 worms for each condition. Data represent one out of three independent experiments.

Back to article page