Supplementary Figure 5: VCP-UBXN10 localizes to cilia. | Nature Cell Biology

Supplementary Figure 5: VCP-UBXN10 localizes to cilia.

From: Systematic proteomics of the VCP–UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis

Supplementary Figure 5

(a) GFP-UBXN10 localizes to cilia in LLC-PK1. Scale bar is 10 μm. (b) Localization of VCP, NPLOC4, UBXN1 and UBXN7 in LLC-PK1 cells. LLC-PK1 cells were infected with virus encoding the indicated GFP tagged proteins. Stable cell lines were serum-starved for 48 h, fixed and stained with acetylated tubulin. Scale bar is 10 μM. (c) The UBX domain of UBXN10 was modeled onto the FAF1-VCP co-crystal structure (PDB ID: 3QWZ) using PHYRE. The location of the TMEVPR loop in UBXN10 is represented.

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