Supplementary Figure 1: Proteomic analysis of the VCP-UBXD adaptor interaction network. | Nature Cell Biology

Supplementary Figure 1: Proteomic analysis of the VCP-UBXD adaptor interaction network.

From: Systematic proteomics of the VCP–UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis

Supplementary Figure 1

(a) Expression of C-HA/FLAG tagged baits in HEK-293T cells and comparison to endogenous proteins. Relative Expression levels were determined by quantification with a CCD camera in a FlourChem-M (Protein Simple). (b) Histograms representing bait abundance (based on average APSM) in α-FLAG and α-HA AP-MS experiments for all baits and the number of HCIPS identified for each bait. n = 4 LC-MS/MS experiments (derived form 2 technical replicates of two biological replicates). Error bars are mean +/− s.e.m from the four replicates. (c) Overlap of HCIPs in 6 AP-MS experiments. For this analysis, we used NWD ≥ 0.98, z ≥ 4, APSM ≥ 2. (d) Gene Ontology (GO) analysis for all unique proteins from Tiers 1-3. Analysis was performed with DAVID (http://david.abcc.ncifcrf.gov/). (e) Co-localization of C-HA/FLAG FAF2 with mitochondria. HeLa cells expressing C-HA/FLAG FAF2 were untreated or treated with 10 μM carbonyl cyanide m-chlorophenyl hydrazone (CCCP) for 4 h. Cells were fixed and stained with α-HA and α-TOMM20. Scale bar is 10 μm.

Back to article page