Supplementary Figure 7: Ectopic NIK expression enhances the in vivo tumorigenicity of C250T GBM cells through p52 activation.

(a) T98G cells were stably transduced with lentiviral expression constructs for vector, NIK WT and NIK WT in combination with shRNA targeting p52 (NIK shp52) and total cell lysates were analyzed by western blotting with the indicated antibodies. Representative image from two independent experiments is shown. Unprocessed original scans of blots are found in Supplementary Fig. 7. (b) 5 NOD-SCID mice were injected subcutaneously with T98G cells expressing vector (red arrows), NIK WT (white arrows) or NIK WT sh-p52 (black arrows) and the tumor growth of these cells in the xenograft mouse model after 10 weeks are depicted. (c) Representative immunohistochemical staining of T98G NIK WT xeonograft tumors with the indicated antibodies. Scale bars, 100 μm. (d) Relative telomerase activity of T98G cells expressing vector, NIK WT or NIK sh-p52. Data shown represent the mean of 3 independent experiments. Error bars represent S.E.M. ^∗P < 0.05; Student’s t-test, two-tailed. All raw data are shown in Supplementary Table 2.