Supplementary Figure 5: Hierarchical clustering analysis of meta-adhesome proteins identified during IAC assembly.

IACs were isolated from K562 cells in biological duplicate after 3, 9 and 32 min incubation with FN-coated beads and analysed by MS (data are from 2 independent experiments; see Supplementary Table 11). Throughout IAC maturation, 1,266 of the 2,412 meta-adhesome proteins were identified and were analysed by unsupervised hierarchical clustering, revealing distinct temporal profiles of protein recruitment to IACs. Quantitative heat map displays mean spectral counts as a proportion of the maximum spectral count for each given protein. Twelve clusters were chosen on the basis of a Pearson correlation threshold greater than 0.8, labelled SA1–12, and are indicated by blue and green bars. Literature-curated adhesome⁴ and consensus adhesome proteins identified in each cluster are indicated by gene name (italic, literature-curated adhesome; regular, consensus adhesome; bold, literature-curated adhesome and consensus adhesome). Literature-curated adhesome proteins that interact with consensus adhesome molecules in interaction network analyses are indicated by an asterisk (see Supplementary Table 7 for details). Clusters are shown alongside corresponding profile plots, with the mean temporal profile for each cluster indicated by a red line. The most significantly overrepresented functional annotations for selected clusters are listed. Full details of enriched functional terms are provided in Supplementary Table 13.