Supplementary Figure 6: Hierarchical clustering analysis of meta-adhesome proteins identified during IAC disassembly.

(a) IACs were isolated from adherent U2OS cells in biological triplicate on nocodazole removal and 5, 10 and 15 min after nocodazole washout to examine changes in IAC composition throughout IAC disruption³². Isolated IACs at each time point were analysed by MS (data are from 3 independent experiments; see Supplementary Table 12). Throughout IAC disassembly, 455 of the 2,412 meta-adhesome proteins were identified and were analysed by unsupervised hierarchical clustering, revealing distinct temporal profiles of protein dissociation from IACs. Quantitative heat map displays mean spectral counts as a proportion of the maximum spectral count for each given protein. Seventeen clusters were chosen on the basis of a Pearson correlation threshold greater than 0.8, labelled SD1–17, and are indicated by blue and green bars. Literature-curated adhesome⁴ and consensus adhesome proteins identified in each cluster are indicated by gene name (italic, literature-curated adhesome; regular, consensus adhesome; bold, literature-curated adhesome and consensus adhesome). Literature-curated adhesome proteins that interact with consensus adhesome molecules in interaction network analyses are indicated by an asterisk (see Supplementary Table 7 for details). Clusters are shown alongside corresponding profile plots, with the mean temporal profile for each cluster indicated by a red line. The most significantly overrepresented functional annotations for selected clusters are listed. Full details of enriched functional terms are provided in Supplementary Table 14. (b,c) Area-proportional Venn diagrams showing the overlap between the meta-adhesome and proteins identified by MS during IAC assembly (b) or IAC disassembly (c). For each set, the total number of proteins (black text) and the number of proteins identified in the consensus adhesome (bold red text) is indicated.