Supplementary Figure 5: Relocalization of repair foci to the nuclear periphery correlates with Rad51 recruitment.
From: Heterochromatic breaks move to the nuclear periphery to continue recombinational repair
(a) Quantitation of the experiment described in Fig. 5a shows the total number of γH2Av foci detected in the middle Z-stack or in the entire nucleus (all Z stacks). (b) IF analysis (top) and quantitation (bottom) of cells expressing mCh-HP1a show HP1a RNAi efficiency. (c) Quantitation of the experiment described in Fig. 5b shows the total number of γH2Av foci after RNAi depletions of the indicated components. Only middle Z-stacks of nuclei were used for this analysis. (d) IF analysis of cells expressing GFP-Mu2, fixed 60 min after IR, and stained for GFP, Rad51 and Koi (nuclear periphery), shows colocalizations between Mu2 and Rad51 at the nuclear periphery (highlighted in the zoomed detail). Ctrl = control. Scale bars = 1 μm. Images are maximum intensity projections (b) or middle Z-stacks (d) of nuclei.
