Supplementary Figure 4: PCR of recombined and unrecombined Plk4 transgene.
From: Transient PLK4 overexpression accelerates tumorigenesis in p53-deficient epidermis
(a) Recombination of the transgene was detected by genomic PCR only in double transgenic mouse at E16.5, P1, adult and in different tumours but not in control mice or CRE negative mice, non-recombined allele was detected by a primer designed specifically to amplify the cells which did not recombine the transgene. ß-actin was used as a loading control.
