Supplementary Figure 5: Regulation of DC Arp2/3- and mDia1-dependent actin nucleation by Cdc42 and RhoA.
From: Innate control of actin nucleation determines two distinct migration behaviours in dendritic cells
(a, b) iDCs were treated with LPS for 30 min, washed and further cultured at 37 °C during 16 h. a. Immunoblot analysis was performed as described in the supplementary experimental procedures. (b) Normalized intensity obtained from data showed in panel a. Graphics show mean individual values. (c) Mean instantaneous speed of iDCs migrating in micro-channels and treated with different doses of ML141 (n = 164, 163, 142, 143 and 39 for iDC, iDC 12 μM, iDC 25 μM, iDC 50 μM and iDC 100 μM respectively). 1 experiment out of 3 is shown. In the box plot the bars include 90% of the points, the center corresponds to the median and the box contains 75% of the data. The Mann-Whitney test was applied for statistical analysis. (d) Analysis by flow cytometry of surface expression of the DC activation marker CD86. iDCs were incubated 30 min with LPS (100 ng/ml), washed and cultured overnight.
