Supplementary Figure 8: Unprocessed full scans of key blots.
From: Innate control of actin nucleation determines two distinct migration behaviours in dendritic cells
(a) Full scans of immunoblots showed in Supplementary Fig. 5a. The whole membrane was divided in two based on the 100 kDa molecular weight marker (left panel). Each blot was sequentially incubated with the indicated primary antibody and the corresponding HRP-conjugated secondary antibody (central panel). For loading controls, the membrane containing the 50 kDa band was incubated sequentially with an anti-tubulin antibody and revealed with the corresponding HRP-conjugated secondary antibody (right panel). (b) Full scans of immunoblots showed in Supplementary Fig. 7g. Blots were obtained as described in a, except that the gel was cut based on the 37 kDa molecular weight marker, allowing detection of phospho-MLC and tubulin in the same run.
