Supplementary Figure 1: HeLa cells express both isoforms of ARPC1 and ARPC5.
From: Isoform diversity in the Arp2/3 complex determines actin filament dynamics
(A) Sequence alignment of human ARPC5 (NP_005708.1) and ARPC5L (NP_112240.1) (top) and ARPC1A (NP_ 006400.2) and ARPC1B (NP_005711.1) (bottom). Pink lines denote residues that are not present in the bovine crystal structure (PDB 1K8K), green line denotes residues involved in the interface between ARPC5 and Arp2. (B) Isoform specific antibodies do not cross react with the opposite endogenous (upper panels) or GFP-tagged (lower panels) ARPC1 or ARPC5 isoforms in HeLa cells. (C) The graphs show the relative abundance of the different ARPC1 or ARPC5 isoforms in a panel of cell lines normalized to their own ARPC2 levels relative to HeLa cells. Error bars represent s.e.m. from n = 3 independent experiments. (D) Immunoblot analysis of HeLa cells treated with siRNA against ARPC2 results in concomitant loss of all other complex subunits.
