Supplementary Figure 2: ARPC1 and ARPC5 isoforms differentially affect actin tail lengths.
From: Isoform diversity in the Arp2/3 complex determines actin filament dynamics
(a) Immunofluorescent images from which insets in Fig. 2b were taken. An image of a HeLa cell treated with siRNA against ARPC2 is also included. The virus is labelled in green and actin (phalloidin) in red. Scale bar is 10 μm. (B) Quantification of actin tail lengths of individual siRNA oligonucleotides from the siGenome pools against ARPC5, ARPC5L, ARPC1A and ARPC1B used in Fig. 2. Data from 3 independent experiments were combined and error bars represent s.e.m. from n = 360 tails. (C) Immunoblot analysis of RNAi treated Lifeact-RFP cells used in Fig. 2d to measure virus speeds. (D) HeLa cells stably expressing GFP-tagged ARPC1 and ARPC5 isoforms were treated with 500 nM Latrunculin A for 20 min before imaging. GFP-tagged isoforms recruited to vaccinia virus (red) were bleached and their recovery was measured (ARPC1B is shown). Scale bar is 5 μm. The graph shows a comparison of the recovery kinetics of photobleached GFP-tagged isoforms. Data from 3 independent experiments were combined and error bars represent s.e.m. for the indicated number of virus (n).
