Supplementary Figure 4: Distribution of β1 integrin after retrograde machinery depletion.
From: Persistent cell migration and adhesion rely on retrograde transport of β1 integrin
(a) Normalized mean cells showing distribution of both non-ligand-bound (mAb13) and ligand-bound (12G10) β1 integrin on HeLa or RPE-1 cells that were plated on crossbow-shaped micropatterns. Images represent n = 15 cells per condition (1 representative of 2 independent experiments). Scale bars = 10 μm. (b) Intensity distribution profiles and normalized images of mean cells showing the surface distribution of ligand-bound β1 integrin (12G10) on RPE-1 cells, migrating on fibronectin-coated micropatterned lines. n = 10 cells per condition in one representative experiment. mean ± s.d. Numbers of independent experiments: ctrl siRNA = 4, synt16 siRNA = 3, Rab6 siRNA = 3, and Rab11 siRNA = 2. Scale bar = 10μm.
