Supplementary Figure 6: Lack of detectable sKitL expression from Steel-Dickie allele.
From: A dynamic niche provides Kit ligand in a stage-specific manner to the earliest thymocyte progenitors
(a) Serum KItL levels in 3-week-old wild-type (+/+; n = 18), Sl/+ (n = 7), Sld/+ (n = 7) and Sl/Sld (n = 4) mice measured by ELISA. Error bars show standard errors. Student’s t-test P values are shown. For source data see Supplementary Table 5. (b) Schematic of the exon 7 floxed Kitl allele. (c) Concentration of sKItL in supernatants of HEK293 cells transfected with the empty MSCV-IRES-GFP vector (N = 6) or MSCV-IRES-GFP vectors encoding Sld KitL (N = 3) and ΔEx7 KitL (N = 3), respectively. Error bars show standard errors. For source data see Supplementary Table 5. (d) Bioassay measuring proliferation of the KitL-dependent MC/9 cell line exposed to the supernatants from (c). Values are relative to MC/9 cells grown under optimal proliferation conditions. Error bars show standard errors. N = 3 for each condition. For source data see Supplementary Table 5. (e) Serum KItL levels in 3 week old control (+/+; n = 6) and KitlΔEx7/ΔEx7 (n = 4) mice measured by ELISA. Error bars show standard errors. Student’s t-test P value is shown. For source data see Supplementary Table 5. (f) Total thymic cellularity of 4–5 weeks old +/+ mice (n = 3) and their Sl/+ littermates (n = 3). Error bars show standard errors. The difference between +/+ and Sl/+ genotypes is not statistically significant (P = 0.71). For source data see Supplementary Table 5. (g) Absolute number of phenotypically defined Flt3 + DN1, Flt3–DN1 and DN2 cells in thymi of mice from (f). Error bars show standard errors. No statistical significant differences were observed (P = 0.21, P = 0.48 and P = 0.35, respectively). For source data see Supplementary Table 5. (h) Total bone marrow cells in mice from (f). Error bars show standard errors. The difference between +/+ and Sl/+ is not statistically significant (P = 0.206). For source data see Supplementary Table 5. (i) Number of cells in the indicated phenotypically defined lymphoid progenitor populations in mice from (f). Values are group averages; error bars show standard errors. P-values relative to the control group are shown. For source data see Supplementary Table 5.
