Supplementary Figure 12: Thymic T cell progenitors and bone marrow lymphoid progenitors FACS analysis.
From: A dynamic niche provides Kit ligand in a stage-specific manner to the earliest thymocyte progenitors
(a) Gating strategy used to define Flt3 + DN1 (Lin − CD4 − CD8α − CD25 − c-KithiFlt3 +), Flt3–DN1 (Lin − CD4 − CD8α − CD25 − c-KithiFlt3 −) and DN2 (Lin − CD4 − CD8α − CD25 + c-KithiFlt3 −) thymocytes in young adult mice (4–6 weeks). (b) Gating strategy used to define lymphoid progenitor populations within the bone marrow live (DAPI −) singlet cell population. The following populations were analysed: NK/T progenitors (LIn–B220 − CD49b − NK1.1 − CD122 +), Lymphoid-biased multi-potent progenitors (LMPPs: Lin − B220 − CD49b − NK1.1 − CD122 − Flt3 + CD127 − c-KithiSca1hi), All Lymphoid Progenitors (ALPs: Lin − B220 − CD49b − NK1.1 − CD122 − Flt3 + CD127 + Ly6D − c-Kitlo), B-Lymphoid Progenitors (BLPs: Lin − B220 − CD49b − NK1.1 − CD122 − Flt3 + CD127 + Ly6D + c-Kit −), Common Lymphoid Progenitors (CLPs: Lin − B220 − CD49b − NK1.1 − CD122 − Flt3 + CD127 + Ly6D − c-KitloSca1lo).
