Supplementary Figure 5: Fog induces endocytosis of Smog and is immobilized on heterologous cells expressing Smog::GFP. | Nature Cell Biology

Supplementary Figure 5: Fog induces endocytosis of Smog and is immobilized on heterologous cells expressing Smog::GFP.

From: Modular activation of Rho1 by GPCR signalling imparts polarized myosin II activation during morphogenesis

Supplementary Figure 5

(a) partial rescue of the smog− elongation phenotype by sqh-driven production of Smog and Smog::GFP. (b) Functional Smog::GFP is detected at the surface of epithelial cells: top orthogonal view lower panels single planes (c) Smog::GFP is also detected in intracellular organelles in mesoderm cells (left top, arrowheads). Loss of zygotic Fog reduces Smog::GFP positive organelles in mesoderm cells (top right) prior to constriction (d) Smog::GFP (green) is detected together with extracellular-injected dextran (magenta) at the surface and in intracellular organelles in the ectoderm during intercalation (top panel). Overexpression of Fog increases intracellular Smog::GFP positive organelles (bottom panel, quantified in (g) where n = number of embryos and ∗∗∗∗∗ is p < 0.000005). Vesicle sizes are often larger in cells over-expressing Fog (d enlarged in right panels). (e) Fog (red) is immobilised on the surface of S2 cells expressing Smog::GFP (green); quantified in (f) n = 68 (GFP positive) and 95 (GFP negative) cells. Error bars are Standard Error of Mean. Scale bars 5 μm.

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