Supplementary Figure 5
From: Dicer1–miR-328–Bace1 signalling controls brown adipose tissue differentiation and function
(a) Average food intake quantified by manual recordings of HFD-fed (n = 15) and HFD + RO5508887-fed (n = 15) mice during the course of the feeding experiment (12 weeks). (b) Glucose-stimulated insulin secretion of HFD-fed (n = 7) versus HFD + RO5508887-fed (n = 7) mice. (c) Percentage of insulin-positive (ins +) cells in pancreatic sections from HFD-fed (n = 6) and HFD + RO5508887-fed (n = 6) mice (left). Insulin staining and H&E counterstaining of pancreatic islets in HFD and HFD + RO5508887-fed mice (right). A representative image is shown. Scale bar, 100 μm. (d) Body composition measured by nuclear magnetic resonance of HFD-fed control (n = 15) versus HFD + RO5508887-fed (n = 14) male C57BL/6 mice. (e) H&E staining of SCAT paraffine section in HFD-fed (n = 5) and HFD + RO5508887-fed (n = 5) mice analyzed in using Zeiss Axiovision 4.2 software and adipocyte area cumulatively plotted in 10 increments. If not stated otherwise, boxplots indicate median ± min/max and an unpaired two-tailed Student’s t-test was used to assess statistical significance. *p < 0.05.
