Supplementary Figure 2

(a) Body weight curves of female, NCD-fed Dicer1^ΔBAT/+ (n = 11; black circle) versus control (n = 11; white circle) or female, HFD-fed Dicer1^ΔBAT/+ (n = 10, black diamond) versus control (n = 13; white diamond) mice. No statistically significant interaction was found using two-way ANOVAs for repeated measures (2WA-RM). (b) Relative expression of indicated genes in BAT of male, NCD-fed Dicer1^ΔBAT/+ mice (n = 7) versus control mice (n = 8).(c) Core body temperature on cold (4 °C) exposure for indicated time points of female, Dicer1^ΔBAT/+ (n = 7) versus control (n = 13) mice determined by rectal thermometry. No statistically significant interaction was found using 2WA-RM. (d) Glucose tolerance test of NCD-fed, male Dicer1^ΔBAT/+ (n = 13) versus control animals (n = 20, left) or NCD-fed, female Dicer1^ΔBAT/+ (n = 14) versus control animals (n = 21, right). A statistically significant interaction was found using 2WA-RM for male (P < 0.01; F = 4.165; Df = 4) mice and a borderline significant interaction for female (P < 0.07; F = 2.222; Df = 4) mice. (e) Insulin tolerance test of NCD-fed, male Dicer1^ΔBAT/+ (n = 13) versus control animals (n = 15, left) or NCD-fed, female Dicer1^ΔBAT/+ (n = 14) versus control animals (n = 16, right). No statistically significant interaction was found using 2WA-RM. (f) Serum insulin level of NCD-fed, male Dicer1^ΔBAT/+ (n = 9) versus control mice (n = 10) and HFD-fed, male Dicer1^ΔBAT/+ (n = 5) versus control mice (n = 8, left) or NCD-fed, female Dicer1^ΔBAT/+ (n = 8) versus control mice (n = 9) and HFD-fed, female Dicer1^ΔBAT/+ (n = 5) versus control mice (n = 12, right). (g) Serum leptin level of NCD-fed, male Dicer1^ΔBAT/+ (n = 10) versus control mice (n = 9) and HFD-fed, male Dicer1^ΔBAT/+ (n = 7) versus control mice (n = 13, left) or NCD-fed, female Dicer1^ΔBAT/+ (n = 9) versus control mice (n = 9) and HFD-fed, female Dicer1^ΔBAT/+(n = 10) versus control mice (n = 11, right). (h) Tissue weight/body weight (BW) ratio of indicated tissues in HFD-fed Dicer1^ΔBAT/+ (Liver, n = 10; BAT, n = 4; SCAT, n = 3; VAT, n = 11) versus control (Liver, n = 18; BAT, n = 4; SCAT, n = 4; VAT, n = 17) mice. (i) Thin-layer chromatography-mediated quantification of lipid species (Chol, cholesterole; FFA, free fatty acids; TG, triglycerides) in indicated tissues of HFD-fed Dicer1^ΔBAT/+ (n = 5) versus control (n = 6) mice. (j) Photomicrograph of indicated tissues in HFD-fed Dicer1^ΔBAT/+ and control mice. A representative image is shown. Scale bar, 100 μm. If not stated otherwise, dots indicate mean ± s.e.m., boxplots indicate median ± min/max and an unpaired two-tailed Student’s t-test was used to assess statistical significance. *p < 0.05, **p < 0.01, ***p < 0.001, ^∗∗∗∗p < 0.0001.