Supplementary Figure 6: The description of N-terminal tail deletion mutants.
From: How the kinetochore couples microtubule force and centromere stretch to move chromosomes
(a) Diagram showing construction of the partial (70 Del) and the whole N-terminal tail deletion (112 Del) mutants. To maintain Ndc80 FRET biosensor protein level, we expressed Ndc80 mutants from the endogenous Ndc80 promoter (left, also see Supplementary Table 3). (b) The PCR using primers in (left) amplified in control, 70 Del, or 112 Del (right). (c) The population of interphase, prometaphase, metaphase, anaphase, telophase, and apoptotic cells in control, 70 Del, and 112 Del mutants. n = 1037 (control), 1103 (70 Del), 1074 (112 Del). The mean values were calculated using the data pooled from 2 independent experiments.
