Supplementary Figure 4: Mechanistic studies of NBR2 regulation of AMPK.
From: LncRNA NBR2 engages a metabolic checkpoint by regulating AMPK under energy stress
(a) Protein lysates were prepared from HEK293T cells transfected with empty vector (EV), NBR2 full length (FL), T4, or T5 fragment expression vectors, and analyzed by Western blotting as indicated. (b) Protein lysates prepared from UMRC2 cells stably expressing EV or NBR2 #1 expression vectors were immunoprecipitated by IgG, LKB1 or Folliculin antibodies, and then were analyzed by Western blotting as indicated. Aliquots of the protein lysates (input) were also analyzed directly. (c) 786-O cells infected with either control shRNA or NBR2 shRNA were cultured in medium containing 0 or 25 mM glucose for 12 hours. Protein lysates were prepared and immunoprecipitated by IgG or LKB1 antibodies, and then were analyzed by Western blotting as indicated. Aliquots of the protein lysates (input) were also analyzed directly. (d) Empty vector (EV) or LKB1-infected Hela cells were transfected with EV or NBR2 #1 expression vectors. Protein lysates were prepared and analyzed by Western blotting as indicated. Unprocessed original scans of blots are shown in Supplemental Fig. 8.
