Supplementary Figure 2: AMPK inactivation by compound C or AMPKα siRNA treatment.
From: LncRNA NBR2 engages a metabolic checkpoint by regulating AMPK under energy stress
(a) MDA-MB-231 cells were treated with 20 μM Compound C in 25 or 0 mM glucose-containing medium for 24 hours, and then subjected to Western blotting analysis to measure AMPK activation. (b) MDA-MB-231 cells transfected with AMPKα or control (Ctrl) siRNA were cultured in 25 or 0 mM glucose-containing medium for 24 hours, and then subjected to Western blotting analysis to measure AMPK activation. Unprocessed original scans of blots are shown in Supplemental Fig. 8.
