Supplementary Figure 3: Role of LGR4 and LGR5 during hepatocyte maturation and differentiation.
From: The RSPO–LGR4/5–ZNRF3/RNF43 module controls liver zonation and size
(a) GS and CK19 co-staining in P2, P10 and P30 control and Lgr4/5dLKO mice. (b) GS + staining quantified in the indicated mice. n = 4 mice (P10 control and P30 control) and 5 mice (all other groups). (c) HNF4α and CK19 co-staining in P2, P10 and P30 control and Lgr4/5dLKO mice. (d) SOX9 staining in P2, P10 and P30 control and Lgr4/5dLKO mice. CV, central vein; PV, portal vein. The images in (a,c,d) are representative for 29 mice, and the stainings were repeated 2 times. Data represent mean ± s.d. ∗∗, P < 0.01; ∗∗∗, P < 0.001; ∗∗∗∗, P < 0.0001; two-tailed unpaired t-test (b) was used. Scale bars, 20 μm.
