Supplementary Figure 4: Lgr4 and Lgr5 control liver zonation.
From: The RSPO–LGR4/5–ZNRF3/RNF43 module controls liver zonation and size
(a) Volcano plot showing pericentral genes (in green), periportal genes (in blue) and genes involved in Wnt signalling (in purple) that are differentially expressed in livers of the indicated mice. n = 5 mice per group. (b) Gene sets downregulated in livers of Lgr4/5dLKO mice compared to control mice. n = 5 mice per group. (c,d) ARG1 (c) and PCK1 (d) staining in the indicated mice. CV, central vein; PV, portal vein. The images in (c,d) are representative for 10 mice, and the stainings were repeated 3 times. Empirical Bayes with Benjamini–Hochberg test (a), and weighted Kolmogorov–Smirnov and Mann–Whitney U-test with Benjamini-Hochberg test (b) were used. Scale bars, 100 μm.
