Supplementary Figure 5: Role of LGR4 and LGR5 in the control of liver growth.
From: The RSPO–LGR4/5–ZNRF3/RNF43 module controls liver zonation and size
(a,b,c) GS, CK19 and Ki67 co-staining in P2 (a), P10 (b) and P30 (c) control and Lgr4/5dLKO mice, showing proliferating hepatocytes (arrowheads). Bottom: Hepatocyte proliferation quantified in liver zones of the indicated mice. n = 4 mice (P30 controls), 5 mice (P2 and P10), and 6 mice (P30 Lgr4/5dLKO). These data involved assessment of 2582 cells (P2 control), 2705 cells (P2 Lgr4/5dLKO), 4217 cells (P10 control), 3911 cells (P10 Lgr4/5dLKO), 2882 cells (P30 control) and 3171 cells (P30 Lgr4/5dLKO) from the indicated mice. (d) Relative liver weight of the indicated mice. n = 5 mice (P2 control) and 6 mice (all other groups). (e) Ki67 staining of the indicated mice at embryonic day (E)16.5. (f) Liver cell proliferation quantified in the indicated mice at E16.5. n = 5 mice (control, Lgr5LKO and Lgr4/5dLKO) and 6 mice (Lgr4LKO). CV, central vein; PV, portal vein. The images in (a–c) and (e) are representative for 30 and 21 mice, respectively, and the stainings were repeated 2 times. Data represent mean ± s.d. ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001; ns, not significant; two-tailed unpaired t-test (a–d, f) was used. Scale bars, 50 μm.
