Supplementary Figure 1: LGR4 and LGR5 expression and mouse model generation.
From: The RSPO–LGR4/5–ZNRF3/RNF43 module controls liver zonation and size
(a) Lgr4 and Lgr5 ISH in liver parenchyma of control mice. The images are representative for 4 mice each. (b) Percent of hepatocytes expressing Lgr4 and Lgr5 in the indicated liver zones. n = 4 mice. These data involved assessment of 3346 cells from the indicated mice. (c) Scheme depicting the generation of Rnf43-LacZ mice. (d) Scheme depicting the generation of liver-specific Lgr4/5dLKO mice by crossing with AlbCre mice. Data represent mean ± s.d. Scale bars, (a) 20 μm and (magnification in a) 10 μm.
