Supplementary Figure 1: Characterization of AKT1(E17K) and AKT2(E17K).
From: Glutathione biosynthesis is a metabolic vulnerability in PI(3)K/Akt-driven breast cancer
(a) Anti-HA immunoprecipitates from serum-starved cells were assayed for their ability to phosphorylate a GSK-3β fusion peptide in vitro (data is representative of two independent experiments). (b) Proliferation of cells grown in the presence of serum and growth factors was determined using the WST-1 assay (data are from one experiment that was independently repeated two times with similar results (Supplementary Table 1)). Unprocessed original scans of blots are shown in Supplementary Fig. 6.
