Supplementary Figure 2: Metabolomic profiling of serum-starved MCF10A cells expressing AKT2(E17K) vs. AKT2.

(a) Unbiased hierarchical clustering of relative metabolite abundances in serum-starved MCF10A cells expressing AKT2(E17K) versus AKT2. (b) Fold changes of metabolite abundances in serum-starved MCF10A cells expressing AKT2(E17K) versus AKT2 (data are from one metabolomics experiment that was independently repeated two times with similar results). (c) Relative abundances of glycolytic intermediates (n = 3 technical replicates from a single independent metabolomics experiment; the experiment was repeated twice with similar results (Supplementary Table 1)). (d) Relative abundances of metabolites in the pentose phosphate pathway (n = 3 technical replicates from a single independent metabolomics experiment; the experiment was repeated twice with similar results (Supplementary Table 1)). (e,f) Incorporation of U-¹³C₅-glutamine into glutamine and GSSG over 1, 3, and 8 h in serum-starved cells (n = 3 technical replicates from a single metabolomics experiment (Supplementary Table 1)). All error bars represent s.e.m. ^∗P < 0.05, ^∗∗P < 0.01 by a two-sided Student’s t-test.