Supplementary Figure 7: Spindle dynamics in HU-treated, ycg1-2 and top2-4 cells. | Nature Cell Biology

Supplementary Figure 7: Spindle dynamics in HU-treated, ycg1-2 and top2-4 cells.

From: The Aurora-B-dependent NoCut checkpoint prevents damage of anaphase bridges after DNA replication stress

Supplementary Figure 7

(a) Anaphase spindle disassembly visualized through tubulin (Tub1) and myosin (Myo1) dynamics in a top2-4 cell at 37 °C (top) and in a wild type cell after HU exposure at 30 °C (bottom). Asterisk marks full contraction of the myosin ring. Arrow points to depolymerizing spindle. Numbers indicate time in minutes. Scale bars are 2 μm. (b) Spindle tracks of wild type and ycg1-2 cells. SPB distance is the linear distance between the two spindle pole bodies (SPBs) at each time point. Mean and SD are shown. Anaphase onset was defined when SPB distance was above 2.5 μm. Arrows mark the time of spindle disassembly. n = 7 cells (wild type); 6 (ycg1-2). (c,d) Visualization of Cin8-GFP and Iqg-GFP dynamics during spindle elongation (Spc42-mCherry) in wild type and ycg1-2 cells; time interval is 1.5 min. All scale bars, 2 μm.

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