Supplementary Figure 3: 3D analysis of embryonic and extra-embryonic lineages in human embryos cultured through implantation stages.
From: Self-organization of the human embryo in the absence of maternal tissues
Related to Figs 3 and 4. Human blastocysts developing in vitro until day 11 were fixed and stained at indicated time points. (a) 3D rendering of DAPI stain overlaid with the centre of OCT4-expressing and GATA6-expressing cells. Dots are projected at the back of the volume. (b) Orthogonal views of embryos at different developmental stages stained for aPKC and OCT4. Note the presence of a small pro-amniotic cavity at day 8–9, which is enlarged as development proceeds. All scale bars, 50 μm.
