Supplementary Figure 1: Fibroblasts aggregation at a distance from melanoma regions contain melanoma particles.
From: Melanoma miRNA trafficking controls tumour primary niche formation
(a–d) Immunofluorescence analysis of sections from four melanoma patients. DAPI-stained nuclei appear in blue. (e) Left: Immunohistochemistry staining (20X magnification) of MITF and MelanA in in situ melanoma sample. Right: Immunofluorescence analysis (63X magnification) of DCT (green) and HMB-45 (red) or FSP-1 (green) and HMB-45 (red). (f) Left: H&E staining (20X magnification) of in situ melanoma. Right: Immunofluorescence analysis (63X magnification) of HMB-45 (red) and DCT (green) or FSP-1 (green). Yellow signal indicates co-localization. (g) Left: H&E staining, immunohistochemistry for MITF and MelanA for in situ melanoma (20X magnification). Right: Immunofluorescence analysis of GPNMB (red) and FSP-1 (green). Yellow signal indicates co-localization of FSP-1 and GPNMB (63X magnification), in dashed square higher magnification image (100x magnification). (h) From left to right in in situ melanoma: H&E staining, immunohistochemistry (20X magnification) for MelanA, immunofluorescence analysis of HMB-45 (red) and DCT (green), HMB-45 (red) and FSP-1 (green), GPNMB (red) and FSP-1 (green). Yellow signal indicates co-localization (63X magnification).
