Supplementary Figure 6: Cell dynamics in HGD lesions.
From: A single dividing cell population with imbalanced fate drives oesophageal tumour growth
(a) Box plots of HGFP fluorescence intensity in HGD from at time points shown (see Fig. 6f for protocol). Box indicates 25th and 75th percentiles, line median and whiskers range. n = 100 cells at 0 h, 277 cells at 24 h, 361 cells at 48 h, 600 cells at 72 h. (b,c) Distribution of cell generations at 48 (black) and 72 (purple) hours, after dox withdrawal in HGD (b) and adjacent epithelium (c) (see Fig. 6f and Supplementary Note 2.3). Error bars are standard deviation. (b) In HGD, at 48 h (n = 4 animals), and 72 h (n = 6 animals). (c) In adjacent epithelium (n = 3 animals both time points). (d) Cell division rates per day inferred from HGFP dilution. Errors are s.e.m. (e–h) Lineage tracing in DEN and Sorafenib treated animals. (e) Clone size distribution 22 days after induction of YFP (protocol shown in Fig. 7a). Points are experimental data (n = 68 clones) with clone size binned in intervals of 5 cells per clone. Lines are model predictions by best-fit parameters and blue area shows standard deviation of the prediction (see Supplementary Note 2.3). (f) Representativeness of clones in HGD. In protocol shown in Fig. 7a animals received a pulse of EdU 1 h prior to being culled. Mean percentage EdU labelling within clones (total 1126 cells) and the entire lesion (total 9792 cells), error bars s.e.m. P = 0.40 by Mann Whitney test (n = 56 clones). (g) Clone size distribution (n = 106 clones) in epithelium adjacent to HGD 13 days after induction of YFP, distinguishing basal and suprabasal cells per clone. Height of bars/points indicates the frequency of clones with the given number of basal and suprabasal cells, as indicated on the axes. Green bars are experimental data, points model predictions by best fit parameters, error bars standard deviations of predicted values (see Supplementary Note 2.3). (h) Cell dynamics in HGD in AhcreERTRosa26flConfetti/wt mice, see Fig. 7e–g. Clone size distributions 21 days after induction. Points are experimental data (n = 101 clones from 5 animals), lines model fits and blue area indicates standard deviation (see Supplementary Note 2.3). Data are binned in intervals of 5 cells per clone. See Supplementary Table 4 for source data for a–c and e–h.
