Supplementary Figure 2: Notch activation converts cycling SCs to quiescent SCs.

(a,b) Relative muscle mass normalized to body weight (a) and mRNA expression in MCs (b). n = 3 animals for each group; data are mean ± s.d.; Two-sample t-test; ^∗P < 0.05. Relative muscle masses to body weights were comparable. (c) IHC staining of Pax7. Scale bar, 50 μm. (d,e) Seven-day-old N1^WT and N1^OE/SC mice were intraperitoneally (i.p.) injected daily with tamoxifen for 3 days. Nicd and nuclear GFP proteins are expressed in the Pax7⁺ cells of N1^OE/SC mice. (d) IHC staining from 10-day-old N1^WT and N1^OE/SC TA muscles. Arrows and arrowheads indicate Pax7⁺GFP⁻p57⁻ and Pax7⁺GFP⁺p57⁺ cells, respectively; scale bar, 25 μm. (e) Quantification of Pax7, GFP, and p57 as in d; n = 3 animals for each group; data are mean ± s.d.