Supplementary Figure 2 | Nature Cell Biology

Supplementary Figure 2

From: Kank2 activates talin, reduces force transduction across integrins and induces central adhesion formation

Supplementary Figure 2

(a) MS intensities of Kank1-4 in the FA-enriched sub-proteome and total proteome of wild-type mouse kidney fibroblasts (mean ± s.d.; n = 3 independent MS measurements). (b) Western blot analysis of Kank2 and β1 integrin (Itgb1) in cells expressing scrambled (shScr) and Kank2-specific shRNAs. Actin served to control protein loading. (c) Western blot analysis of Kank2 before and after shRNA-mediated knockdown using polyclonal anti-Kank2, and of indicated GFP-tagged Kank2 constructs using anti-GFP antibodies, respectively. Vinculin is used as loading control.

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