Supplementary Figure 3 | Nature Cell Biology

Supplementary Figure 3

From: Kank2 activates talin, reduces force transduction across integrins and induces central adhesion formation

Supplementary Figure 3

(a,b) Immunofluorescence of GFP-tagged Kank2 constructs (green) co-stained for Liprin-β1 (a, red) or ELKS (b, red), Paxillin (Pxn; blue) and DAPI (grey). (c) Line profile analysis of GFP-tagged FL-Kank2 co-stained with Liprin-β1 and ELKS. Full arrowheads indicate the position of FA belt and open arrowheads indicate Liprin-β1- and ELKS-enriched regions outside the FA belt. Scale bar, 2 μm. (d,e) Western blot testing GFP-tagged Kank-KN (d), FL-Kank2 and Kank2ΔKN (e) binding to biotinylated β1 integrin tails. FL and mutant Kank2 proteins were detected with the anti-GFP antibody, and Kindlin-2 was used to control the β1 tail pull-down assay. (f) GFP-tagged Kank1, Kank3 and Kank4 expressed in Kank2-depleted fibroblasts and co-stained for Paxillin (Pxn; red), F-actin (blue) and DAPI (grey). Scale bars in a,b,f, 10 μm.

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